Split-luciferase Complementation Imaging Assay to Study Protein-protein Interactions in Nicotiana benthamiana.

ABSTRACT: The experimental identification of protein-protein interactions (PPIs) is critical to understand protein function. Thus, a plethora of sensitive and versatile approaches have been developed to detect PPIs in vitro or in vivo, such as protein pull-down, yeast two-hybrid (Y2H), co-immunoprecipitation (co-IP), and bimolecular fluorescence complementation (BiFC) assays. The recently established split-luciferase complementation (Split-LUC) imaging assay has several advantages compared to other approaches to detect PPIs in planta: it is a relatively simple and fast method to detect PPIs in vivo; the results are quantitative, with high sensitivity and low background; it measures dynamic PPIs in real-time; and it requires limited experimental materials and instrumentati

SUBMITTER: Wang L 

PROVIDER: S-EPMC8678545 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature