The Dawn of next generation DNA sequencing in myelodysplastic syndromes- experience from Pakistan.

ABSTRACT:

Background

Myelodysplastic syndromes (MDS) are clonal disorders of hematopoietic stem cells exhibiting ineffective hematopoiesis and tendency for transformation into acute myeloid leukemia (AML). The available karyotyping and fluorescent in situ hybridization provide limited information on molecular abnormalities for diagnosis/prognosis of MDS. Next generation DNA sequencing (NGS), providing deep insights into molecular mechanisms being involved in pathophysiology, was employed to study MDS in Pakistani cohort.

Patients and methods

It was a descriptive cross-sectional study carried out at National institute of blood diseases and bone marrow transplant from 2016 to 2019. Total of 22 cases of MDS were included. Complete blood counts, bone marrow assessment and cytogenetic anal

SUBMITTER: Anwar N 

PROVIDER: S-EPMC8679965 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature