Novel high-resolution targeted sequencing of the cervicovaginal microbiome.

ABSTRACT:

Background

The cervicovaginal microbiome (CVM) plays a significant role in women's cervical health and disease. Microbial alterations at the species level and characteristic community state types (CST) have been associated with acquisition and persistence of high-risk human papillomavirus (hrHPV) infections that may result in progression of cervical lesions to malignancy. Current sequencing methods, especially most commonly used multiplex 16S rRNA gene sequencing, struggle to fully clarify these changes because they generally fail to provide sufficient taxonomic resolution to adequately perform species-level associative studies. To improve CVM species designation, we designed a novel sequencing tool targeting microbes at the species taxonomic rank and examined its potential for pro

SUBMITTER: Andralojc KM 

PROVIDER: S-EPMC8680041 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature