A new method for obtaining bankable and expandable adult-like microglia in mice.

ABSTRACT:

Background

The emerging role of microglia in neurological disorders requires a novel method for obtaining massive amounts of adult microglia. We aim to develop a new method for obtaining bankable and expandable adult-like microglia in mice.

Methods

The head neuroepithelial layer (NEL) that composed of microglial progenitor and neuroepithelial cells at mouse E13.5 was dissected and then cultured or banked. Microglia (MG) isolated from the cultured NEL by magnetic-activated cell sorting system were obtained and named NEL-MG.

Results

The NEL included microglia progenitors that proliferate and ramify over time with neuroepithelial cells as feeder. In functional analysis, NEL-MG exhibited microglial functions, such as phagocytosis (microbeads, amyloid β, synaptosome), migr

SUBMITTER: You MJ 

PROVIDER: S-EPMC8680120 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature