Accurate prediction of acute pancreatitis severity based on genome-wide cell free DNA methylation profiles.

ABSTRACT:

Background

Patients with severe acute pancreatitis (SAP) have a high mortality, thus early diagnosis and interventions are critical for improving survival. However, conventional tests are limited in acute pancreatitis (AP) stratification. We aimed to assess AP severity by integrating the informative clinical measurements with cell free DNA (cfDNA) methylation markers.

Methods

One hundred and seventy-five blood samples were collected from 61 AP patients at multiple time points, plus 24 samples from healthy individuals. Genome-wide cfDNA methylation profiles of all samples were characterized with reduced representative bisulfite sequencing. Clinical blood tests covering 93 biomarkers were performed on AP patients within 24 h. SAP predication models were built based on cfDNA me

SUBMITTER: Sun HW 

PROVIDER: S-EPMC8680202 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature