Division of labor between SOS and PafBC in mycobacterial DNA repair and mutagenesis.

ABSTRACT: DNA repair systems allow microbes to survive in diverse environments that compromise chromosomal integrity. Pathogens such as Mycobacterium tuberculosis must contend with the genotoxic host environment, which generates the mutations that underlie antibiotic resistance. Mycobacteria encode the widely distributed SOS pathway, governed by the LexA repressor, but also encode PafBC, a positive regulator of the transcriptional DNA damage response (DDR). Although the transcriptional outputs of these systems have been characterized, their full functional division of labor in survival and mutagenesis is unknown. Here, we specifically ablate the PafBC or SOS pathways, alone and in combination, and test their relative contributions to repair. We find that SOS and PafBC have both distinct and overlapp

SUBMITTER: Adefisayo OO 

PROVIDER: S-EPMC8682763 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature