Mouse microRNA signatures in joint ageing and post-traumatic osteoarthritis.

ABSTRACT:

Objective

This study investigated mice serum and joint microRNA expression profiles in ageing and osteoarthritis to elucidate the role of microRNAs in the development and progression of disease, and provide biomarkers for ageing and osteoarthritis.

Design

Whole joints and serum samples were collected from C57BL6/J male mice and subjected to small RNA sequencing. Groups used included; surgically-induced post-traumatic osteoarthritis, (DMM; 24 months-old); sham surgery (24 months-old); old mice (18 months-old); and young mice (8 months-old). Differentially expressed microRNAs between the four groups were identified and validated using real-time quantitative PCR. MicroRNA differential expression data was used for target prediction and pathway analysis.

Results

In joint t

SUBMITTER: Castanheira CIGD 

PROVIDER: S-EPMC8683752 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature