Fragment antigen binding domains (F_abs) as tools to study assembly-line polyketide synthases.

ABSTRACT: The crystallization of proteins remains a bottleneck in our fundamental understanding of their functions. Therefore, discovering tools that aid crystallization is crucial. In this review, the versatility of fragment-antigen binding domains (F_abs) as protein crystallization chaperones is discussed. F_abs have aided the crystallization of membrane-bound and soluble proteins as well as RNA. The ability to bind three F_abs onto a single protein target has demonstrated their potential for crystallization of challenging proteins. We describe a high-throughput workflow for identifying F_abs to aid the crystallization of a protein of interest (POI) by leveraging phage display technologies and differential scanning fluorimetry (DSF). This workflow has proven

SUBMITTER: Guzman KM 

PROVIDER: S-EPMC8683866 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature