M2 macrophages secrete CXCL13 to promote renal cell carcinoma migration, invasion, and EMT.

ABSTRACT:

Objective

M2 macrophages are associated with a poor prognosis in a variety of malignancies. There are, however, few relevant investigations in clear cell renal cell carcinoma (ccRCC).

Methods

The expression of M2 macrophages in ccRCC tissues was first discovered using immunohistochemistry in this study. Then, M2 macrophages were created in vitro to see how they affected the proliferation, migration, invasion, and EMT of ccRCC cells. Using qPCR and prognostic analysis identifies important chemokine. Antibody neutralization tests confirmed the chemokine's involvement and function. Pathway inhibitors confirmed the main pathway of M2 macrophages in ccRCC. Finally, qPCR and IHC were used to confirm the expression of chemokine receptors in ccRCC tissues.

Results

The presenc

SUBMITTER: Xie Y 

PROVIDER: S-EPMC8684162 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature