Transcriptome analysis of ovary culture-induced embryogenesis in cucumber (Cucumis sativus L.).

ABSTRACT:

Background

Ovary culture is a useful technique used to generate double haploid (DH) cucumber (Cucumis sativus L.) plants. However, cucumber ovary culture have a low rate of embryo induction and plant regeneration. Moreover, the cucumber embryogenesis mechanism remains unclear. In this study, we explored the molecular basis of cucumber embryogenesis in order to establish a foundation for a more efficient ovary culture method. Using transcriptome sequencing, we also investigated the differential expression of genes during the embryogenesis process.

Methods

Cytological and morphological observations have divided cucumber ovary culture into three stages: early embryo development (T0), embryo morphogenesis (T1, T2, T3 and T4), and shoot formation (T5). We selected six key

SUBMITTER: Deng Y 

PROVIDER: S-EPMC8684322 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature