Genomic characterization of triple-carbapenemase-producing Acinetobacter baumannii.

ABSTRACT:

Objectives

To characterize Acinetobacter baumannii OCU_Ac16a, a clinical isolate co-harbouring three acquired carbapenemase genes, bla _NDM-1, bla _TMB-1, and bla _OXA-58, and assess the clinical significance of so-called multiple-carbapenemase producers.

Methods

OCU_Ac16a and its close relative, OCU_Ac16b, which lacks the bla _NDM-1, were isolated from sputum cultures of a patient at Osaka City University Hospital. We subjected these strains to whole-genome analysis, particularly focusing on the genetic context of each carbapenemase gene. The transmissibility and functionality of each carbapenemase gene were analysed by conjugation and transformation experiments and antimicrobial susceptibility tests

SUBMITTER: Oinuma KI 

PROVIDER: S-EPMC8684466 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature