Dataset Information

The Impact of Long-term Physical Inactivity on Adipose Tissue Immunometabolism.

ABSTRACT:

Context

Adipose tissue and physical inactivity both influence metabolic health and systemic inflammation, but how adipose tissue responds to chronic physical inactivity is unknown.

Objective

This work aimed to characterize the impact of chronic physical inactivity on adipose tissue in healthy, young males.

Methods

We collected subcutaneous adipose tissue from 20 healthy, young men before and after 60 days of complete bed rest with energy intake reduced to maintain energy balance and fat mass. We used RNA sequencing, flow cytometry, ex vivo tissue culture, and targeted protein analyses to examine adipose tissue phenotype.