InMut-finder: a software tool for insertion identification in mutagenesis using Nanopore long reads.

ABSTRACT:

Background

Biological mutagens (such as transposon) with sequences inserted, play a crucial role to link observed phenotype and genotype in reverse genetic studies. For this reason, accurate and efficient software tools for identifying insertion sites based on the analysis of sequencing reads are desired.

Results

We developed a bioinformatics tool, a Finder, to identify genome-wide Insertions in Mutagenesis (named as "InMut-Finder"), based on target sequences and flanking sequences from long reads, such as Oxford Nanopore Sequencing. InMut-Finder succeeded in identify > 100 insertion sites in Medicago truncatula and soybean mutants based on sequencing reads of whole-genome DNA or enriched insertion-site DNA fragments. Insertion sites discovered by InMut-Finder were validated

SUBMITTER: Song R 

PROVIDER: S-EPMC8684674 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature