Modeling a disease-correlated tubulin mutation in budding yeast reveals insight into MAP-mediated dynein function.

ABSTRACT: Mutations in the genes that encode α- and β-tubulin underlie many neurological diseases, most notably malformations in cortical development. In addition to revealing the molecular basis for disease etiology, studying such mutations can provide insight into microtubule function and the role of the large family of microtubule effectors. In this study, we use budding yeast to model one such mutation-Gly436Arg in α-tubulin, which is causative of malformations in cortical development-in order to understand how it impacts microtubule function in a simple eukaryotic system. Using a combination of in vitro and in vivo methodologies, including live cell imaging and electron tomography, we find that the mutant tubulin is incorporated into microtubules, causes a shift in α-tubulin isotype usage, and

SUBMITTER: Denarier E 

PROVIDER: S-EPMC8684761 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature