Functional Restoration of Bacteriomes and Viromes by Fecal Microbiota Transplantation.

ABSTRACT:

Background & aims

Fecal microbiota transplantation (FMT) is an effective therapy for recurrent Clostridioides difficile infection (rCDI). However, the overall mechanisms underlying FMT success await comprehensive elucidation, and the safety of FMT has recently become a serious concern because of the occurrence of drug-resistant bacteremia transmitted by FMT. We investigated whether functional restoration of the bacteriomes and viromes by FMT could be an indicator of successful FMT.

Methods

The human intestinal bacteriomes and viromes from 9 patients with rCDI who had undergone successful FMT and their donors were analyzed. Prophage-based and CRISPR spacer-based host bacteria-phage associations in samples from recipients before and after FMT and in donor samples were examined

SUBMITTER: Fujimoto K 

PROVIDER: S-EPMC8684800 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature