Transcriptome-wide N ⁶-Methyladenosine Methylome Profiling Reveals m⁶A Regulation of Skeletal Myoblast Differentiation in Cattle (Bos taurus).

ABSTRACT: N ⁶ -methyladenosine (m⁶A) is the most prevalent methylation modification of eukaryotic mRNA, and it plays an important role in regulating gene expression. Previous studies have found that m6A methylation plays a role in mammalian skeletal muscle development. However, the effect of m⁶A on bovine skeletal myogenesis are still unclear. Here, we selected proliferating myoblasts (GM) and differentiated myotubes (on the 4th day of differentiation, DM) for m⁶A-seq and RNA-seq to explore the m⁶A methylation modification pattern during bovine skeletal myogenesis. m⁶A-seq analysis revealed that m⁶A methylation was an abundant modification of the mRNA in bovine myoblasts and myotubes. We scanned 5,691-8,094 m<

SUBMITTER: Yang X 

PROVIDER: S-EPMC8685427 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature