[Identification and validation of hub genes in prostate cancer progression based on weighted gene co-expression network analysis].

ABSTRACT:

Objective

To identify the key hub genes in prostate cancer metastasis based on weighted gene co-expression network analysis (WGCNA) and verify the identified genes.

Methods

Whole-genome chip data GSE6919 of prostate cancer study were analyzed using principal component analysis (PCA), and the differentially expressed genes (DEGs) were analyzed using R software. WGCNA was performed to construct a gene co-expression network for screening the key genes. TCGA database was used to explore the expressions of the DEGs and their association with the prognosis. To validate the results, we designed siRNA fragments targeting the metastasis-related gene HNRNPA2B1, and observed its effect on growth, apoptosis, clone formation, migration and invasion of prostate cancer cell lines us

SUBMITTER: Zhang H 

PROVIDER: S-EPMC8685695 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature