Endocytic proteins with prion-like domains form viscoelastic condensates that enable membrane remodeling.

ABSTRACT: Membrane invagination and vesicle formation are key steps in endocytosis and cellular trafficking. Here, we show that endocytic coat proteins with prion-like domains (PLDs) form hemispherical puncta in the budding yeast, Saccharomyces cerevisiae These puncta have the hallmarks of biomolecular condensates and organize proteins at the membrane for actin-dependent endocytosis. They also enable membrane remodeling to drive actin-independent endocytosis. The puncta, which we refer to as endocytic condensates, form and dissolve reversibly in response to changes in temperature and solution conditions. We find that endocytic condensates are organized around dynamic protein-protein interaction networks, which involve interactions among PLDs with high glutamine contents. The endocytic coat pr

SUBMITTER: Bergeron-Sandoval LP 

PROVIDER: S-EPMC8685726 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature