Dataset Information

Evolution of variable lymphocyte receptor B antibody loci in jawless vertebrates.

ABSTRACT: Three types of variable lymphocyte receptor (VLR) genes, VLRA, VLRB, and VLRC, encode antigen recognition receptors in the extant jawless vertebrates, lampreys and hagfish. The somatically diversified repertoires of these VLRs are generated by serial stepwise copying of leucine-rich repeat (LRR) sequences into an incomplete germline VLR gene. Lymphocytes that express VLRA or VLRC are T cell-like, while VLRB-expressing cells are B cell-like. Here, we analyze the composition of the VLRB locus in different jawless vertebrates to elucidate its configuration and evolutionary modification. The incomplete germline VLRB genes of two hagfish species contain short noncoding intervening sequences, whereas germline VLRB genes in six representative lamprey species have much longer intervening sequences that exhibit notable genomic variation. Genomic clusters of potential LRR cassette donors, fragments of which are copied to complete VLRB gene assembly, are identified in Japanese lamprey and sea lamprey. In the sea lamprey, 428 LRR cassettes are located in five clusters spread over a total of 1.7 Mbp of chromosomal DNA. Preferential usage of the different donor cassettes for VLRB assemblage is characterized in our analysis, which reveals evolutionary modifications of the lamprey VLRB genes, elucidates the organization of the complex VLRB locus, and provides a comprehensive catalog of donor VLRB cassettes in sea lamprey and Japanese lamprey.

SUBMITTER: Das S

PROVIDER: S-EPMC8685922 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Evolution of variable lymphocyte receptor B antibody loci in jawless vertebrates.

Das Sabyasachi S Rast Jonathan P JP Li Jianxu J Kadota Mitsutaka M Donald John A JA Kuraku Shigehiro S Hirano Masayuki M Cooper Max D MD

Proceedings of the National Academy of Sciences of the United States of America 20211201 50

Three types of variable lymphocyte receptor (VLR) genes, VLRA, VLRB, and VLRC, encode antigen recognition receptors in the extant jawless vertebrates, lampreys and hagfish. The somatically diversified repertoires of these VLRs are generated by serial stepwise copying of leucine-rich repeat (LRR) sequences into an incomplete germline VLR gene. Lymphocytes that express VLRA or VLRC are T cell-like, while VLRB-expressing cells are B cell-like. Here, we analyze the compos ...[more]

PMID: 34880135

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Dataset Information

Evolution of variable lymphocyte receptor B antibody loci in jawless vertebrates.

Publications

Evolution of variable lymphocyte receptor B antibody loci in jawless vertebrates.

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