Rapid Emergence of T Follicular Helper and Germinal Center B Cells Following Antiretroviral Therapy in Advanced HIV Disease.

ABSTRACT: Low nadir CD4 T-cell counts in HIV⁺ patients are associated with high morbidity and mortality and lasting immune dysfunction, even after antiretroviral therapy (ART). The early events of immune recovery of T cells and B cells in severely lymphopenic HIV⁺ patients have not been fully characterized. In a cohort of lymphopenic (CD4 T-cell count < 100/µL) HIV⁺ patients, we studied mononuclear cells isolated from peripheral blood (PB) and lymph nodes (LN) pre-ART (n = 40) and 6-8 weeks post-ART (n = 30) with evaluation of cellular immunophenotypes; histology on LN sections; functionality of circulating T follicular helper (cTfh) cells; transcriptional and B-cell receptor profile on unfractionated LN and PB samples; and plasma biomarker measurements. A group of 1

SUBMITTER: Wong CS 

PROVIDER: S-EPMC8686113 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature