Multiview confocal super-resolution microscopy.

ABSTRACT: Confocal microscopy¹ remains a major workhorse in biomedical optical microscopy owing to its reliability and flexibility in imaging various samples, but suffers from substantial point spread function anisotropy, diffraction-limited resolution, depth-dependent degradation in scattering samples and volumetric bleaching². Here we address these problems, enhancing confocal microscopy performance from the sub-micrometre to millimetre spatial scale and the millisecond to hour temporal scale, improving both lateral and axial resolution more than twofold while simultaneously reducing phototoxicity. We achieve these gains using an integrated, four-pronged approach: (1) developing compact line scanners that enable sensitive, rapid, diffraction-limited imaging over large areas;

SUBMITTER: Wu Y 

PROVIDER: S-EPMC8686173 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature