Ontology highlight
ABSTRACT:
SUBMITTER: Yang JM
PROVIDER: S-EPMC8686192 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Yang Jr-Ming JM Chi Wei-Yu WY Liang Jessica J Takayanagi Saki S Iglesias Pablo A PA Huang Chuan-Hsiang CH
Cell 20211126 25
Genetically encoded fluorescent biosensors are powerful tools for monitoring biochemical activities in live cells, but their multiplexing capacity is limited by the available spectral space. We overcome this problem by developing a set of barcoding proteins that can generate over 100 barcodes and are spectrally separable from commonly used biosensors. Mixtures of barcoded cells expressing different biosensors are simultaneously imaged and analyzed by deep learning models to achieve massively mul ...[more]