Influence of basal media composition on barrier fidelity within human pluripotent stem cell-derived blood-brain barrier models.

ABSTRACT: It is increasingly recognized that brain microvascular endothelial cells (BMECs), the principal component of the blood-brain barrier (BBB), are highly sensitive to soluble cues from both the bloodstream and the brain. This concept extends in vitro, where the extracellular milieu can also influence BBB properties in cultured cells. However, the extent to which baseline culture conditions can affect BBB properties in vitro remains unclear, which has implications for model variability and reproducibility, as well as downstream assessments of molecular transport and disease phenotypes. Here, we explore this concept by examining BBB properties within human-induced pluripotent stem cell (iPSC)-derived BMEC-like cells cultured under serum-free conditions in DMEM/F12 and Neurobasal media, which ha

SUBMITTER: Neal EH 

PROVIDER: S-EPMC8688328 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature