Chemokines modulate glycan binding and the immunoregulatory activity of galectins.

ABSTRACT: Galectins are versatile glycan-binding proteins involved in immunomodulation. Evidence suggests that galectins can control the immunoregulatory function of cytokines and chemokines through direct binding. Here, we report on an inverse mechanism in which chemokines control the immunomodulatory functions of galectins. We show the existence of several specific galectin-chemokine binding pairs, including galectin-1/CXCL4. NMR analyses show that CXCL4 binding induces changes in the galectin-1 carbohydrate binding site. Consequently, CXCL4 alters the glycan-binding affinity and specificity of galectin-1. Regarding immunomodulation, CXCL4 significantly increases the apoptotic activity of galectin-1 on activated CD8⁺ T cells, while no effect is observed in CD4⁺ T cells. The o

SUBMITTER: Sanjurjo L 

PROVIDER: S-EPMC8688422 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature