Deconvoluting virome-wide antibody epitope reactivity profiles.

ABSTRACT:

Background

Comprehensive characterization of exposures and immune responses to viral infections is critical to a basic understanding of human health and disease. We previously developed the VirScan system, a programmable phage-display technology for profiling antibody binding to a library of peptides designed to span the human virome. Previous VirScan analytical approaches did not carefully account for antibody cross-reactivity among sequences shared by related viruses or for the disproportionate representation of individual viruses in the library.

Methods

Here we present the AntiViral Antibody Response Deconvolution Algorithm (AVARDA), a multi-module software package for analyzing VirScan datasets. AVARDA provides a probabilistic assessment of infection with species-level r

SUBMITTER: Monaco DR 

PROVIDER: S-EPMC8688874 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature