Efficient production of immunologically active Shigella invasion plasmid antigens IpaB and IpaH using a cell-free expression system.

ABSTRACT: Shigella spp. invade the colonic epithelium and cause bacillary dysentery in humans. Individuals living in areas that lack access to clean water and sanitation are the most affected. Even though infection can be treated with antibiotics, Shigella antimicrobial drug resistance complicates clinical management. Despite decades of effort, there are no licensed vaccines to prevent shigellosis. The highly conserved invasion plasmid antigens (Ipa), which are components of the Shigella type III secretion system, participate in bacterial epithelial cell invasion and have been pursued as vaccine targets. However, expression and purification of these proteins in conventional cell-based systems have been challenging due to solubility issues and extremely low recovery yields. These difficulties have im

SUBMITTER: Kapoor N 

PROVIDER: S-EPMC8688910 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature