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Intrinsic fluorescence-based label-free detection of bovine amyloid A amyloidosis.

ABSTRACT: Amyloidosis is diagnosed by the histologic detection of amyloid deposits; however, this method has limitations such as a prolonged diagnosis time and the need for histologic proficiency. We aimed to develop a rapid and simple method for diagnosing amyloidosis by targeting amyloid-specific endogenous fluorescence, which has not been reported previously, to our knowledge. Fluorescence fingerprint analysis of amyloid extracts and tissue homogenates derived from amyloid A (AA) amyloidosis-affected cattle exhibited a specific intrinsic fluorescence pattern. Furthermore, principal component analysis using analytical data revealed that AA could be identified by peaks near λ_ex 350 nm and λ_em 430 nm. Fluorescence spectrometry analysis using tissue homogenates, which does not require special histochemical staining, enables the rapid detection of bovine AA.

SUBMITTER: Ujike N

PROVIDER: S-EPMC8689041 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Intrinsic fluorescence-based label-free detection of bovine amyloid A amyloidosis.

Ujike Naoki N Iwaide Susumu S Ono Yuki Y Okano Takayuki T Murakami Tomoaki T

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 20211005 1

Amyloidosis is diagnosed by the histologic detection of amyloid deposits; however, this method has limitations such as a prolonged diagnosis time and the need for histologic proficiency. We aimed to develop a rapid and simple method for diagnosing amyloidosis by targeting amyloid-specific endogenous fluorescence, which has not been reported previously, to our knowledge. Fluorescence fingerprint analysis of amyloid extracts and tissue homogenates derived from amyloid A (AA) amyloidosis-affected c ...[more]

PMID: 34609271

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