Application of microfluidic systems in modelling impacts of environmental structure on stress-sensing by individual microbial cells.

ABSTRACT: Environmental structure describes physical structure that can determine heterogenous spatial distribution of biotic and abiotic (nutrients, stressors etc.) components of a microorganism's microenvironment. This study investigated the impact of micrometre-scale structure on microbial stress sensing, using yeast cells exposed to copper in microfluidic devices comprising either complex soil-like architectures or simplified environmental structures. In the soil micromodels, the responses of individual cells to inflowing medium supplemented with high copper (using cells expressing a copper-responsive pCUP1-reporter fusion) could be described neither by spatial metrics developed to quantify proximity to environmental structures and surrounding space, nor by computational modelling of flui

SUBMITTER: Harvey HJ 

PROVIDER: S-EPMC8689086 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature