Harnessing galactose oxidase in the development of a chemoenzymatic platform for glycoconjugate vaccine design.

ABSTRACT: In the preparation of commercial conjugate vaccines, capsular polysaccharides (CPSs) must undergo chemical modification to generate the reactive groups necessary for covalent attachment to a protein carrier. One of the most common approaches employed for this derivatization is sodium periodate (NaIO₄) oxidation of vicinal diols found within CPS structures. This procedure is largely random and structurally damaging, potentially resulting in significant changes in the CPS structure and therefore its antigenicity. Additionally, periodate activation of CPS often gives rise to heterogeneous conjugate vaccine products with variable efficacy. Here, we explore the use of an alternative agent, galactose oxidase (GOase) isolated from Fusarium sp. in a chemoenzymatic approach to generate a

SUBMITTER: Duke JA 

PROVIDER: S-EPMC8689215 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature