Trochanteric Bursa Is a Source of Connective Tissue Progenitor Cells.

ABSTRACT:

Purpose

To investigate the presence of connective tissue progenitor cells (CTPs) in the trochanteric bursa harvested over the gluteus medius muscle belly and tendon during open hip procedures.

Methods

Trochanteric bursa samples from nine patients (63.1 ± 8.6 years) undergoing total hip arthroplasty for primary osteoarthritis were obtained from 2 sites: over the gluteus medius tendon at the greater trochanter and over the muscle belly. Bursal tissue was digested with collagenase and grown in culture. The nucleated cell count, cellular concentration, cellular proliferation, fluorescence-activated cell sorting (FACS) analysis, and differentiation using immunostaining and quantitative polymerase chain reaction (PCR) were used to determine and quantify the presence of CTPs.

Re

SUBMITTER: LeVasseur MR 

PROVIDER: S-EPMC8689227 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature