The tryptophan pathway and nicotinamide supplementation in ischaemic acute kidney injury.

ABSTRACT:

Background

Down-regulation of the enzymes involved in tryptophan-derived nicotinamide (NAM) adenine dinucleotide (NAD⁺) production was identified after acute kidney injury (AKI), leading to the hypothesis that supplementation with NAM may increase the kidney NAD⁺ content, rescuing tryptophan pathways and subsequently improving kidney outcomes.

Methods

Urinary measurement of tryptophan and kynurenin using liquid chromatography-mass spectrometry metabolomics was used in a cohort of 167 cardiac bypass surgery patients along with tests for correlation to the development of postoperative AKI. A mouse model of ischaemic AKI using ischaemia-reperfusion injury (bilateral clamping of renal arteries for 25 min) was also used.

Results

We identified a signif

SUBMITTER: Piedrafita A 

PROVIDER: S-EPMC8690092 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature