Novel clonality assays for T cell lymphoma in cats targeting the T cell receptor beta, T cell receptor delta, and T cell receptor gamma loci.

ABSTRACT:

Background

T cell clonality assays in veterinary medicine currently target only the T cell receptor gamma (TRG) locus. Existing assays have suboptimal sensitivity because of insufficient primer coverage of all possible rearrangements.

Objective

Develop higher sensitivity clonality assays targeting the TRG, delta (TRD), and beta (TRB) loci in cats.

Animals

Cats with histopathologically confirmed lymphoma (n = 89), non-lymphoma (n = 35), and possible hepatic small cell lymphoma (n = 31).

Methods

Molecular clonality assay development utilizing our recently reported topology and expressed repertoire data of the T cell receptor loci in cats. Determination of clonality status of lymphoma, non lymphoma, and possible hepatic small cell lymphoma samples, and calculation

SUBMITTER: Radtanakatikanon A 

PROVIDER: S-EPMC8692208 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature