Analysis of muscle tissue in vivo using fiber-optic autofluorescence and diffuse reflectance spectroscopy.

ABSTRACT:

Significance

Current methods for analyzing pathological muscle tissue are time consuming and rarely quantitative, and they involve invasive biopsies. Faster and less invasive diagnosis of muscle disease may be achievable using marker-free in vivo optical sensing methods.

Aim

It was speculated that changes in the biochemical composition and structure of muscle associated with pathology could be measured quantitatively using visible wavelength optical spectroscopy techniques enabling automated classification.

Approach

A fiber-optic autofluorescence (AF) and diffuse reflectance (DR) spectroscopy device was manufactured. The device and data processing techniques based on principal component analysis were validated using in situ measurements on healthy skeletal and cardiac

SUBMITTER: Davey CJ 

PROVIDER: S-EPMC8692235 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature