Comparison of HPLC and NMR for quantification of the main volatile fatty acids in rumen digesta.

ABSTRACT: Accurate quantification of volatile fatty acid (VFA) concentrations in rumen fluid are essential for research on rumen metabolism. The study comprehensively investigated the pros and cons of High-performance liquid chromatography (HPLC) and ¹H Nuclear magnetic resonance (¹H-NMR) analysis methods for rumen VFAs quantification. We also investigated the performance of several commonly used data pre-treatments for the two sets of data using correlation analysis, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The molar proportion and reliability analysis demonstrated that the two approaches produce highly consistent VFA concentrations. In the pre-processing of NMR spectra, line broadening and shim correction may reduce estimate

SUBMITTER: Wang M 

PROVIDER: S-EPMC8692319 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature