Development of an in vitro tissue culture system for hammer coral (Fimbriaphyllia ancora) ovaries.

ABSTRACT: In vitro gonad culture systems have proven useful to investigate intrinsic mechanisms of sexual reproduction in animals. Here we describe development of an in vitro culture method for coral ovaries. Mesenterial tissues containing both ovaries and mesenterial filaments were microscopically isolated from the scleractinian coral, Fimbriaphyllia ancora, and culture conditions were optimized. M199 diluted 10× (10% M199, pH 8.1) and supplemented with 25 mM HEPES and the antibiotics, ampicillin, penicillin and streptomycin, supported oocyte survival and maintained the structural integrity of ovaries during short-term culture (~ 6 days). Addition of a commercial antibiotic-antimycotic solution (Anti-Anti) and fetal bovine serum adversely affected ovary maintenance and caused tissue disintegration.

SUBMITTER: Chiu YL 

PROVIDER: S-EPMC8692509 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature