Biosynthetic potential of uncultured Antarctic soil bacteria revealed through long-read metagenomic sequencing.

ABSTRACT: The growing problem of antibiotic resistance has led to the exploration of uncultured bacteria as potential sources of new antimicrobials. PCR amplicon analyses and short-read sequencing studies of samples from different environments have reported evidence of high biosynthetic gene cluster (BGC) diversity in metagenomes, indicating their potential for producing novel and useful compounds. However, recovering full-length BGC sequences from uncultivated bacteria remains a challenge due to the technological restraints of short-read sequencing, thus making assessment of BGC diversity difficult. Here, long-read sequencing and genome mining were used to recover >1400 mostly full-length BGCs that demonstrate the rich diversity of BGCs from uncultivated lineages present in soil from Mars Oasis, An

SUBMITTER: Waschulin V 

PROVIDER: S-EPMC8692599 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature