Aptamer Probes Labeled with Lanthanide-Doped Carbon Nanodots Permit Dual-Modal Fluorescence and Mass Cytometric Imaging.

ABSTRACT: High-dimensional imaging mass cytometry (IMC) enables simultaneous quantification of over 35 biomarkers on one tissue section. However, its limited resolution and ultralow acquisition speed remain major issues for general clinical application. Meanwhile, conventional immunofluorescence microscopy (IFM) allows sub-micrometer resolution and rapid identification of the region of interest (ROI), but only operates with low multiplicity. Herein, a series of lanthanide-doped blue-, green-, and red-fluorescent carbon nanodots (namely, B-Cdots(Ln₁ ), G-Cdots(Ln₂ ), and R-Cdots(Ln₃ )) as fluorescence and mass dual-modal tags are developed. Coupled with aptamers, B-Cdots(¹⁵⁹ Tb)-A10-3.2, G-Cdots(¹⁶⁵ Ho)-AS1411, and R-Cdots(¹⁶⁹ Tm)-SYL3

SUBMITTER: Yu Y 

PROVIDER: S-EPMC8693039 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature