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Identification and enhancing production of a novel macrolide compound in engineered Streptomyces peucetius.

ABSTRACT: Streptomyces peucetius produces doxorubicin and daunorubicin, which are important anticancer drugs. In this study, we activate peucemycin, a new antibacterial compound, using an OSMAC strategy. In general, bioactive compounds are produced in a higher amount at room temperature; however, in this study, we have demonstrated that a bioactive novel compound was successfully activated at a low temperature (18 °C) in S. peucetius DM07. Through LC-MS/MS, IR spectroscopy, and NMR analysis, we identified the structure of this compound as a γ-pyrone macrolide. This compound was found to be novel, thus named peucemycin. It is an unusual 14-membered macrocyclic γ-pyrone ring with cyclization. Also, peucemycin exhibits potential antibacterial activity and a suppressive effect on the viability of various cancer cell lines.

SUBMITTER: Pham VTT

PROVIDER: S-EPMC8693821 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Identification and enhancing production of a novel macrolide compound in engineered Streptomyces peucetius.

Pham Van Thuy Thi VTT Nguyen Hue Thi HT Nguyen Chung Thanh CT Choi Ye Seul YS Dhakal Dipesh D Kim Tae-Su TS Jung Hye Jin HJ Yamaguchi Tokutaro T Sohng Jae Kyung JK

RSC advances 20210114 5

Streptomyces peucetius produces doxorubicin and daunorubicin, which are important anticancer drugs. In this study, we activate peucemycin, a new antibacterial compound, using an OSMAC strategy. In general, bioactive compounds are produced in a higher amount at room temperature; however, in this study, we have demonstrated that a bioactive novel compound was successfully activated at a low temperature (18 °C) in S. peucetius DM07. Through LC-MS/MS, IR spectroscopy, and NMR analysis, ...[more]

PMID: 35424263

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