Comparison of Four Carbapenemase Detection Methods for bla_KPC-2 Variants.

ABSTRACT: Recently, various bla_KPC-2 variants resistant to ceftazidime-avibactam have begun to emerge in clinical settings, but it is unclear which testing method is most appropriate for detecting these variants. Strains were subjected to antimicrobial susceptibility testing using the broth microdilution method. Four carbapenemase detection methods, modified carbapenem inactivation method (mCIM) and EDTA carbapenem inactivation method (eCIM), APB/EDTA (carbapenemase inhibitor APB [3-aminophenylboronic acid] and EDTA enhancement method), NG-test Carba 5, and GeneXpert Carba-R were used to try to detect KPC-2 variants in 19 Klebsiella pneumoniae isolates. Among those bla_KPC-2 variants, bla_KPC-33-, bla_KPC-35-, bla_KPC-71

SUBMITTER: Ding L 

PROVIDER: S-EPMC8693920 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature