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Self-Assembled Peptide-Labeled Probes for Agglutination-Based Sensing.

ABSTRACT: The use of polydiacetylene (PDA) vesicles in sensing systems are wide-spread due to the interesting optical properties of this stimuli-responsive material; however, agglutination based sensing with PDA have been relatively underutilized. To demonstrate the means for rapidly generating an agglutination probe based on peptide-displaying polydiacetylene vesicles, we implement here the use of a biotin mimetic peptide functionalized to a diacetylene amphiphile for proof-of-concept detection of a multivalent target, specifically streptavidin. Tuning of the vesicle composition revealed a distinct limit in the surface density of peptide amphiphile that could be displayed for this particular peptide sequence. A wide operational detection range was demonstrated, and the result also revealed an effective agglutination response of the PDA-based probe to streptavidin suggesting possible use of future formulations in profiling other multivalent targets.

SUBMITTER: Shrestha A

PROVIDER: S-EPMC8693994 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Self-Assembled Peptide-Labeled Probes for Agglutination-Based Sensing.

Shrestha Anura A Lim Butaek B Shiveshwarkar Priyanka P Rojas Gumaro G Abure Izele I Nelson Anthony David AD Jaworski Justyn J

Macromolecular research 20210925 9

The use of polydiacetylene (PDA) vesicles in sensing systems are wide-spread due to the interesting optical properties of this stimuli-responsive material; however, agglutination based sensing with PDA have been relatively underutilized. To demonstrate the means for rapidly generating an agglutination probe based on peptide-displaying polydiacetylene vesicles, we implement here the use of a biotin mimetic peptide functionalized to a diacetylene amphiphile for proof-of-concept detection of a mult ...[more]

PMID: 34955698

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