A label-free fluorescent peptide probe for sensitive and selective determination of copper and sulfide ions in aqueous systems.

ABSTRACT: A label free fluorescent peptide probe (HDSGWEVHH) was used for Cu²⁺ and S^2- determination in aqueous solution. Our results demonstrated that HDSGWEVHH is highly selective and sensitive for monitoring free Cu²⁺ concentration via quenching of the probe fluorescence upon Cu²⁺ binding. The mechanism of the complexation is investigated with Cyclic Voltammetry (CV), ¹H nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR) spectroscopy and computational techniques. Theoretical calculation results indicated the binding ratio of the probe to Cu²⁺ is 2 : 1 and the binding constant was obtained as 1.72 × 10 ⁸ M^-1. Cu²⁺ concentration can be detected with the detection limit of 1

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8694936 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature