A straightforward and sensitive "ON-OFF" fluorescence immunoassay based on silicon-assisted surface enhanced fluorescence.

ABSTRACT: A straightforward immunoassay based on silicon-assisted surface enhanced fluorescence (SEF) has been demonstrated using a silicon-based fluorescent immune substrate and silver-antibody nanoconjugate (SANC). The P-doped, (100) oriented silicon wafers are used for both fluorophore attachment and antigen immobilization. The silicon substrate offers a very low blank signal in the "OFF" state, due to its fluorescence quenching effect. In the detection process, the capture of the SANCs by the surface-immobilized antigen leads to an effectively enhanced fluorescence to produce an "ON" state. The analytical performance of the presented scheme has been investigated and a limit of detection of 31.4 pg mL^-1 has been obtained. Besides the broadened application range compared with the conven

SUBMITTER: Zhang R 

PROVIDER: S-EPMC8695005 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature