Optimisation of bacterial release from a stable microfluidic-generated water-in-oil-in-water emulsion.

ABSTRACT: Application of droplet microfluidics for the encapsulation of bacteria in water-in-oil-in-water (W/O/W) emulsion allows for production of monodisperse droplets with controllable size. In this study the release of bacteria from W/O/W emulsion, the effect of the double emulsion structure on bacterial growth and metabolic activity, and the stability and mechanism of bacterial release were investigated. W/O/W emulsions were formed using a double flow-focusing junction microfluidic device under controlled pressure to produce droplets of approximately 100 μm in diameter containing an inner aqueous phase (W₁) of about 40-50 μm in diameter. GFP-labelled Escherichia coli (E. coli-GFP) bacteria were encapsulated within the W₁ droplets and the stability of emulsion

SUBMITTER: Mohd Isa NS 

PROVIDER: S-EPMC8695039 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature