[Cytotoxicity and underlying mechanism of evodiamine in HepG2 cells].

ABSTRACT:

Objective

To investigate evodiamine (EVO)-induced hepatotoxicity and the underlying mechanism.

Methods

HepG2 cells were treated with EVO (0.04-25 μmol/L) for different time intervals, and the cell survival rate was examined by cell counting kit-8 (CCK-8) method. After HepG2 cells were treated with EVO (0.2, 1 and 5 μmol/L) for 48 h, the alanine transaminase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP) activities and total bilirubin (TBIL) content of supernatant were detected. A multifunctional microplate reader was used to detect the intracellular superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in HepG2 cells to evaluate the level of cell lipid peroxidation damage. The interactions between EVO and apop

SUBMITTER: Gao YD 

PROVIDER: S-EPMC8695168 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature