On-line separation/analysis of Rhodamine B dye based on a solid-phase extraction high performance liquid chromatography self-designed device.

ABSTRACT: A special self-designed device based on poly-1-vinyl-3-pentylimidazole hexafluorophosphate (PILs-C₅) solid-phase extraction and high performance liquid chromatography (HPLC) is proposed as a novel method for the on-line separation and analysis of Rhodamine B (RhB) dye. Single factor experiment design and orthogonal experiment design were used to optimize the experimental parameters, such as pH, the amount of PILs-C₅, sample volume, flow rate, eluent type, eluent concentration, eluent volume, and the flow rate of eluent. Under the optimized conditions, the linear range was 0.02-2.4 μg mL^-1, with the correlation coefficients (R ²) of 0.997. The limit of detection (LOD) and limit of quantification (LOQ) were 0.004 μg mL^-1 and 0.02

SUBMITTER: Wu J 

PROVIDER: S-EPMC8695180 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature