Development of a colorless Centella asiatica (L.) Urb. extract using a natural deep eutectic solvent (NADES) and microwave-assisted extraction (MAE) optimized by response surface methodology.

ABSTRACT: This study outlines a green process for Centella asiatica (L.) Urb. (CA) extraction. Natural deep eutectic solvents (NADESs) and microwave-assisted extraction (MAE) were combined to provide a high bioactive compound yield and high antioxidant activity. Among the NADESs evaluated, the combination of acetylcholine chloride : malic acid : water (1 : 2 : 2): water (40 : 60) was the best for extraction. These conditions provide high madecassoside (MS) (21.7 mg g^-1 dry weight) and asiaticoside (AS) (12.7 mg g^-1 dry weight) yields, with greater than 80% (v/v) EtOH (13.3 mg g^-1 MS and 7.80 mg g^-1 AS). In addition, the extracts from this process showed higher antioxidant activity (IC₅₀ = 0.26 mg mL^-1) than the CA aqueous EtOH

SUBMITTER: Phaisan S 

PROVIDER: S-EPMC8695212 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature