A conserved mechanism for regulating replisome disassembly in eukaryotes.

ABSTRACT: Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by ubiquitylation of the CDC45-MCM-GINS (CMG) replicative helicase^1-3. Despite being driven by evolutionarily diverse E3 ubiquitin ligases in different eukaryotes (SCF^Dia2 in budding yeast¹, CUL2^LRR1 in metazoa^4-7), replisome disassembly is governed by a common regulatory principle, in which ubiquitylation of CMG is suppressed before replication termination, to prevent replication fork collapse. Recent evidence suggests that this suppression is mediated by replication fork DNA^8-10. However, it is unknown how SCF^Dia2 and CUL2^LRR1 discriminate terminated from elongating replisomes, to selectively ubiquitylate CMG only a

SUBMITTER: Jenkyn-Bedford M 

PROVIDER: S-EPMC8695382 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature