Monitoring the heme iron state in horseradish peroxidase to detect ultratrace amounts of hydrogen peroxide in alcohols.

ABSTRACT: Despite the importance of hydrogen peroxide (H₂O₂) in initiating oxidative damage and its connection to various diseases, the detection of low concentrations of H₂O₂ (<10 μM) is still limited using current methods, particularly in non-aqueous systems. One of the most common methods is based on examining the color change of a reducing substrate upon oxidation using UV/Vis spectrophotometry, fluorophotometry and/or paper test strips. In this study, we show that this method encounters low efficiency and sensitivity for detection of ultratrace amounts of H₂O₂ in non-aqueous media. Thus, we have developed a simple, fast, accurate and inexpensive method based on UV/Vis spectrophotometry to detect H₂O₂ in non-

SUBMITTER: Ravanfar R 

PROVIDER: S-EPMC8695524 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature