Duplex PCR assay for detection of chickpea chlorotic dwarf virus and peanut witches' broom phytoplasma in chickpea.

ABSTRACT: A duplex PCR assay was standardized by optimizing PCR reaction constituents and cycles for the simultaneous detection of chickpea chlorotic dwarf virus (CpCDV) and a peanut witches' broom (PnWB) phytoplasma associated with the chickpea stunt disease. Coat protein gene and tuf gene specific primers for CpCDV and phytoplasmas were used. Different concentrations of the PCR components such as Taq polymerase, primers and PCR annealing temperature were standardized for the identification of the two agents by a duplex PCR assay. Expected amplicons of 590 bp for CpCDV and 1090 bp for phytoplasmas were consistently amplified from the symptomatic chickpea tissues. That resulted in equally efficient and sensitive in detecting single or mixed infection of CpCDV and PnWB phytoplasma in 14

SUBMITTER: Reddy MG 

PROVIDER: S-EPMC8695644 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature