Nasopharyngeal carcinoma related miRNA detection through a DSN enzyme assisted tetrahedral probe for more accurate prognosis.

ABSTRACT: MicroRNAs (miRNAs) play a pivotal role in cellular functions and in the development and progression of cancer. Precise quantification of miRNAs from different clinical samples is a challenging but necessary endeavor that is largely neglected by many emerging fluorescence technologies. Herein, we propose here a novel miRNA detection method through a designed tetrahedral probe and DSN enzyme for attached signal amplification. The highlights of the method are calculated as: (i) the tetrahedral probe induced a much higher transfection efficiency than normal probes; (ii) the DSN enzyme-based cleavage ensures amplification and thus leads to a high detection sensitivity. In all, we believe that the proposed method could greatly improve the sensitivity of intracellular miRNA imaging methods and co

SUBMITTER: Zhang L 

PROVIDER: S-EPMC8695942 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature